Plasmid

Part:BBa_K3779004

Designed by: JiaCheng, Li   Group: iGEM21_NWU-CHINA-B   (2021-06-19)


pPICZαA

pPICZα is a terminal fusion vector. To express your gene as a recombinant fusion protein, you must clone your gene in frame with the N-terminal α-factor secretion signal and the C-terminal peptide containing the c-myc epitope and the polyhistidine tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 1677
    Illegal XhoI site found at 1184
    Illegal XhoI site found at 1246
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1224
    Illegal NgoMIV site found at 2435
    Illegal NgoMIV site found at 2496
    Illegal AgeI site found at 1396
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1660
    Illegal BsaI.rc site found at 1859
    Illegal BsaI.rc site found at 2857


[edit]
Categories
//awards/composite_part
Parameters
chassis